解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The differentiation of body-wall muscle cells was studied in the nematode Caenorhabditis elegans. Specific antibodies to myosin and paramyosin, major protein constituents of differentiated muscle, react with mesodermal cells in wild-type embryos towards the end of the first half of embryogenesis. Immunoreactive cells ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90025-3
更新日期:1982-08-01 00:00:00
abstract::Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the presence of an extract of pituitary gland. Adipogenic activity is found in standard growth hormone preparations derived from different species. Further purification of rat growth hormone by several methods does not remove its adi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90440-8
更新日期:1982-07-01 00:00:00
abstract::Sensory transduction in E. coli consists of two phases, excitation and adaptation, both of which involve the methyl-accepting chemotaxis proteins (MCPs). These molecules relay transmembrane signals and are reversibly methylated during adaptation of E. coli to environmental stimuli. Each MCP contains multiple sites of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90438-x
更新日期:1982-07-01 00:00:00
abstract::The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains has been investigated. Seven independent mutations at the white locus were examined, and these fell into two classes on the basis of their genetic and structural properties. The five mutations comprising the f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90462-7
更新日期:1982-07-01 00:00:00
abstract::Xenopus laevis oocytes respond to high temperature (greater than 31 degrees C) by the synthesis of one major (70 kilodalton) protein and by a gradual reduction in the rate of normal protein synthesis. In contrast with most other cells, the heat-shock response of Xenopus oocytes is controlled exclusively at the transla...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90443-3
更新日期:1982-07-01 00:00:00
abstract::A basic B-cell-specific protein of 35,000 daltons (XM1) is expressed at a high rate--comparable to actin--in resting splenic B lymphocytes of various species. Splenic or thymic T cells or fibroblasts do not express this protein. Upon polyclonal stimulation of B lymphocytes or in proliferating B-lymphocyte-derived cell...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90162-3
更新日期:1982-06-01 00:00:00
abstract::Strains of Saccharomyces that carry the nib allele of a nuclear gene exhibit a "nibbled" colony morphology if they also harbor the plasmid 2 mu DNA. I have found that the expression of the nibbled phenotype is correlated with the presence of a subpopulation of abnormally large cells that give rise to mortal clones. La...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90174-x
更新日期:1982-06-01 00:00:00
abstract::A novel method of affinity chromatography on insolubilized collagen-binding fragments of fibronectin was utilized to isolate a random-coil collagenous protein from culture media of mouse teratocarcinoma-derived endodermal cells. These cells also produced another collagenous protein, which did not bind to fibronectin b...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90164-7
更新日期:1982-06-01 00:00:00
abstract::Genetic and transcriptional maps have been constructed for the mitochondrial genome of the Ascomycete Podospora anserina. These data have been plotted on the restriction maps for Sal I, Xho I, Bam HI, Eco RI, BgI II and Hae III. We have characterized and cloned a new and unique senescent mitochondrial DNA (beta-event ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90167-2
更新日期:1982-06-01 00:00:00
abstract::Transcription of U1 small nuclear RNA from a 592 bp fragment of human DNA was analyzed in vivo and in vitro. When injected into Xenopus laevis oocyte nuclei, the cloned DNA is transcribed by RNA polymerase II to make human U1 snRNA. Thus the sequences of this fragment are sufficient for expression of the U1 snRNA gene...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90111-8
更新日期:1982-05-01 00:00:00
abstract::The small ribonucleoproteins recognized by anti-La autoantibodies contain a heterogeneous mixture of small RNAs from uninfected mammalian cells. The identity of many of these has now been established by the discovery of precursor forms of 5S rRNA and of certain tRNAs among La RNAs from HeLa cells. The small fraction o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90099-x
更新日期:1982-05-01 00:00:00
abstract::We studied the chromatin structure and methylation of ribosomal RNA genes (rDNA) in hybrids between Xenopus laevis and Xenopus borealis. S1-nuclease protection experiments showed that 97%-98% of the rRNA precursor in hybrid tadpoles was of the X. laevis type. Preferential expression of the laevis rDNA was correlated w...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90105-2
更新日期:1982-05-01 00:00:00
abstract::As visualized by electron microscopy, RecA protein binds in a highly cooperative manner to single-stranded fd DNA in solutions of 0.01 M Tris (pH 7.5). The resulting nucleoprotein filament loops are 1.25 micrometers in length, have a fiber diameter of 12 nm and show an indication of a 4.5 nm repeat along the axis of t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90055-1
更新日期:1982-04-01 00:00:00
abstract::We have established the DNA sequence of nine yeast mutants that prevent the expression either of the split cytochrome b gene alone (five mutants) or of two split genes, the cytochrome b gene and the cytochrome oxidase subunit I gene (four mutants). All the mutations analyzed are localized in intron 14 of the cob-box g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90051-4
更新日期:1982-04-01 00:00:00
abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90221-5
更新日期:1982-03-01 00:00:00
abstract::In target tissues for estrogen, including breast cancer cells, the synthesis of progesterone receptors (PRs) is controlled by estradiol acting through estrogen receptors (ERs). We describe studies with T47D human breast cancer cells, whose PRs are not regulated by estradiol, though present in extraordinary amounts (30...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90218-5
更新日期:1982-03-01 00:00:00
abstract::We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but ra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90202-1
更新日期:1982-03-01 00:00:00
abstract::Five single base pair mutations that increase expression of the tnpA (transposase) gene of the Tn3 transposon approximately 30-fold, but which still allow the gene to be regulated, have been isolated by using a generally applicable procedure that involves distally linked lac gene fusions. The mutations, which are all ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90352-x
更新日期:1982-02-01 00:00:00
abstract::We describe a monoclonal antibody directed against a neuron-specific mitochondrial protein from rat brain. On protein blots the antibody recognizes a single polypeptide of apparent molecular weight of 23,000. By solid-phase immunoassay the antigen was detected in nonneural tissues. Within neurons, the antibody stains ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90343-9
更新日期:1982-02-01 00:00:00
abstract::Transposon Tn10 inserts at many sites in the bacterial chromosome, but preferentially inserts at particular hotspots. We believe we have identified the target DNA signal responsible for this specificity. We have determined the DNA sequences of 11 Tn10 insertion sites and identified a particular 6 base pair (bp) symmet...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90385-3
更新日期:1982-01-01 00:00:00
abstract::Rodent cells resistant to PALA, a specific inhibitor of the aspartate transcarbamylase activity of the multifunctional CAD protein, overproduce CAD as a result of amplification of the CAD gene. We cloned a functional CAD gene from Syrian hamster cells using a cosmid vector. Two independently isolated cosmids containin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90410-4
更新日期:1981-12-01 00:00:00
abstract::In cells of the B-lymphocyte lineage, 8.4 kb transcripts are constitutively produced from unrearranged kappa constant region (kappa 0) loci. To help elucidate the molecular basis of this phenomenon, we have determined the nucleotide sequence surrounding the site of transcriptional initiation. The kappa 0 transcripts a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90401-3
更新日期:1981-12-01 00:00:00
abstract::We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly sp...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90387-1
更新日期:1981-12-01 00:00:00
abstract::For the faithful initiation of transcription of exogenously added fibroin gene, a crude cellular extract was prepared from the posterior silk glands where fibroin gene is specifically transcribed. The faithful initiation was demonstrated by the assay with use of truncated fibroin gene templates and confirmed by nuclea...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90371-8
更新日期:1981-11-01 00:00:00
abstract::The tyrT locus of E. coli contains a 208 bp spacer region that separates two copies of sequence encoding tRNATyr1. The spacer includes a 120 bp sequence that is homologous to a sequence that is repeated three times in the distal portion of the tyrT locus. The tyrT locus possesses a graded set of transcription terminat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90199-9
更新日期:1981-11-01 00:00:00
abstract::Drosophila chorion genes are located in two clusters, one on the X chromosome at 7F1-2 of the polytene chromosome map and a second on the third chromosome at 66D11-15. Genes in both regions undergo amplification in ovarian follicle cells prior to their expression late in oogenesis. Analysis of cloned genomic segments ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90373-1
更新日期:1981-11-01 00:00:00
abstract::An examination of Abelson murine leukemia virus (A-MuL V)-hematopoietic cell interaction in cultures of fetal tissues reveals that A-MuLV can stimulate the formation of two different types of colonies. One type of colony is white and composed of A-MuLV-transformed lymphoid cells that can develop into established cell ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90035-0
更新日期:1981-10-01 00:00:00
abstract::Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90308-1
更新日期:1981-10-01 00:00:00
abstract::We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90028-3
更新日期:1981-10-01 00:00:00
abstract::The proportion of BFU-E normally engaged in DNA synthesis is low in adult B6 (C57BL/6) mice of genotype Fv2rr (resistant to Friend erythroleukemia virus), as shown by 3H-thymidine or hydroxyurea "cell suicide" experiments in vivo and vitro. When bone marrow cells from these mice were subjected to a single wash in alph...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90306-8
更新日期:1981-10-01 00:00:00
abstract::Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90185-9
更新日期:1981-09-01 00:00:00
abstract::A hybrid beta-galactosidase molecule containing a substantial portion of the amino-terminal sequence of the maltose-binding protein is inserted in the cytoplasmic membrane of E. coli; in this location, the protein has very low enzymatic activity. The strain producing it is, therefore, Lac-. Selection for derivatives o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90184-7
更新日期:1981-09-01 00:00:00
abstract::Recombinant DNA techniques have been used to isolate the integrated viral DNA, together with flanking cellular sequences, from three independently isolated lines of SV40-transformed BALB/c 3T3 cells. We have determined the structures of these cloned segments and their relationships to one another by restriction enzyme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90073-8
更新日期:1981-08-01 00:00:00
abstract::Recent work has identified a cascade of membrane bound protein kinases in Ehrlich ascites tumor cells. These enzymes, designated PKL, PKS and PKM, are present in both Ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. We have now purified a fourth protein kinase, PKF, that is also assoc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90227-0
更新日期:1981-07-01 00:00:00
abstract::M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90099-4
更新日期:1981-06-01 00:00:00
abstract::We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90092-1
更新日期:1981-06-01 00:00:00
abstract::The modulation of cell metabolism by cell shape and external surface contact has been studied by suspension culture of anchorage-dependent fibroblasts. The suspended cells shut down protein synthesis and nuclear RNA metabolism and cease replicating DNA. However, these responses to suspension are lost or modified as ce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90111-2
更新日期:1981-06-01 00:00:00
abstract::tDNA sequences essential for promotion of transcription have been identified for a tRNA1met gene of X. laevis. A cloned tRNA gene unit was altered by resection of the 5' flanking sequences or by specific deletions of gene and trailing sequences. Gene internal sequences were also substituted by unrelated sequences. The...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90348-2
更新日期:1981-05-01 00:00:00
abstract::The chromosomal distributions of five families of mouse r-protein genes (S16, L18, L19, L30 and L32/33) were studied by Southern blot analysis of DNa from a panel of mouse-hamster hybrid cells containing various complements of mouse chromosomes. Our results indicated that members of a particular family are often locat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90320-2
更新日期:1981-05-01 00:00:00
abstract::The copy number of yeast histone genes was increased by inserting an extra H2A,H2B gene pair into the haploid genome by the technique of yeast transformation. The presence of this extra gene copy has no detectable effect on cell growth. The steady-state levels of histone H2A,H2B mRNAs are not elevated in transformed s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90327-5
更新日期:1981-05-01 00:00:00